Research methods in biology
On the exam, you may be asked to discuss, evaluate, or contrast research methods used to study the brain and behaviour, hormones and/or pheromones, or genetics.
When discussing methods, it is important to think about both how the method is used and why this choice is made.
For each of the studies below, see if you can identify the research method that was used.
Task 1: Which method was used?
The following list of studies are studies used throughout the course. For each one, think about which method was used and then think about which of three questions above would you be able to answer with this study: brain and behaviour, hormones/pheromones, or genetics.
1. Newcomer's study on the role of cortisol on declarative memory.
True experiment.
Area of assessment: The role of hormones on behaviour.
2. Milner and Corkin's study of HM.
A longitudinal case study.
Area of assessment: the brain and behaviour.
3. Wedekind's Smelly T-shirt study
A quasi-experiment. The study is repeated measures but different individuals in the study have different MHC profiles.
Area of assessment: the role of genetics on behaviour (evolutionary theories fit under this heading).
4. Maguire's Taxi cab driver study.
A natural experiment study that made use of brain imaging technology. You could also argue that it is a quasi experiment. The individuals were not randomly allocated to groups (you are either a taxi driver or you are not). So, the study is quasi-experimental. The question is, "who manipulates the variable?" In this case, it is naturally occurring. So, a natural experiment is perhaps the better answer. The IB accepts both quasi and natural experiments to describe this study.
Area of assessment: the brain and behaviour.
5. Meany's study of the role of glucocorticoids on memory.
A true experiment.
Area of assessment: the brain and behaviour.
6. Marmot's Whitehall study to see the role of social hierarchies on health of British workers in the Civil Service.
Case study.
Area of assessment: the role of hormones on behaviour.
7. Rogers & Kesner's study of the role of acetylcholine on memory formation in rats.
A true experiment.
Area of assessment: the brain and behaviour.
8. Caspi's study of the role of 5HTT on depression
A natural experiment. Individuals cannot be randomly allocated to conditions because the study is based on the participants' phenotypes. The occurrence of stressful life events is not manipulated by the researcher; they are naturally occurring.
Area of assessment: the role of genetics on behaviour
9. Buss's cross-cultural study of what people look for in a mate.
A questionnaire.
Area of assessment: the role of genetics on behaviour
10. Sharot's study of brain activity and flashbulb memory
A quasi experiment. Participants were allocated to groups based on where they were on 9/11. The IV (the type of words that were shown to the participants) was manipulated by the researcher.
Area of assessment: the brain and behaviour
11. Kiecolt and Glaser's study of stress and health in first-year medical students.
A natural experiment. Participants were first identified for their stress levels before the examination session, but the exam session was not manipulated by the researchers, it was naturally occurring.
Area of assessment: the role of hormones on behaviour.
12. Bailey and Pillard's study of homosexuality in MZ and DZ twins.
This could be considered either a correlation or a natural experiment.
Area of assessment: the role of genetics on behaviour
Experimental method: how
First, a very brief overview of experimental design.
When writing about the nature of experimental research, there are some key traits of the method that you should be able to discuss.
- Hypothesis testing - experiments propose a hypothesis and then create a situation in which to test whether or not that hypothesis can be supported.
- Variables - An experiment manipulates an independent variable and measures the effect on a dependent variable. Some experiments have more than one IV and DV. Other variables, called extraneous or confounding variables, are controlled - or at least there is an attempt at controlling them so that they do not affect the outcome of the experiment. Control of variables raises internal validity and helps to establish a cause-and-effect relationship.
- A big question in experiments is "who manipulates the independent variable?" When the researcher does it and participants are randomly allocated to groups, this is a "true" experiment. If participants are not randomly allocated, this is a quasi-experiment. If the research does not manipulate the IV, this is a natural experiment.
- Random allocation - it is important that participants are randomly allocated to conditions. When done in a large enough sample, random allocation is an effective way to reduce bias.
- A representative sample should be obtained. This means that the sample should be large enough and consist of participants that reflect the nature of the population being studied.
- Experiments can be done in a laboratory or in a more natural environment. When done in the "real world", these are called field experiments. Obviously, field experiments have less control over variables than lab experiments.
- Experiments use designs - for example, a repeated measures or independent samples design. In a repeated measures design, to make sure that the order of the conditions is not influencing the results, counter-balancing is often used - that is, some participants are given condition 1 first and then condition 2; other participants are given condition 2 and then condition 1.
Experiments in the biological approach
- One assumption of the biological approach is that animals may be used to give us insight into human behaviour. Of the three approaches that we study, only biologists use animals in their research. This, of course, leads to the problem of generalizability.
- Biologists often use double-blind testing - that is, neither the participants nor the researchers know which participants belong to the control group or the test group. Only after all data have been recorded do the researchers learn which participants were which. Performing an experiment in double-blind fashion can greatly lessen biases that may distort the results.
- Placebos are often used in order to determine whether just getting a treatment was enough to change behaviour.
- Matched pairs are also often used. This is when participants are allocated to groups based on their characteristics so that all groups to be tested have a relatively equal distribution of a variable. In the Whitehall study, for example, participants were matched for smoking. So, smokers were compared at different levels of the hierarchy to make sure that smoking, rather than their place in the social hierarchy, was not the reason for their level of cardiovascular health.
Task 2: Thinking about how
Read the study done by Troster and Beatty (1989). Try to answer each of the following questions in order to apply your knowledge of "how" an experiment is carried out to explain the decisions made by the researchers.
1. Which design was used by the researchers?
The researchers used a repeated measures design. Each participant took part in all three conditions.
2. What was the independent variable?
The independent variable was the level of acetylcholine in the synapse. This was manipulated by giving participants either a placebo or scopolamine (low or high dose), an antagonist for acetylcholine.
3. What were the dependent variables?
The dependent variables were the number of words recalled, locations correctly identified on a map, and the percentage of faces correctly identified.
4. What controls were used in this study?
The placebo group is a control. The researcher wanted to make sure that the simple act of getting an injection did not actually influence their performance. In addition, the study was counter-balanced, so they did not all start with the placebo or with the high level of scopolamine, but also took part in all three conditions.
5. Was the sample appropriate to the study?
The sample was all male, so this may have some influence on the results. Perhaps, for example, men are worse at remembering faces in general compared to women. But the range of ages is good and makes the male sample more generalizable
Experimental method: why
There are several reasons why researchers use experiments. The most significant is the ability to establish a cause and effect relationship. Since the testing conditions are tightly controlled, a strong argument can be made that the change in the IV led to the change in the DV. Experiments are used because we want to eliminate extraneous variables and focus on the role of the IV.
Biologists often employ a reductionist approach. Because of this, experimental research that looks to isolate variables is a logical choice of method.
Experiments also are highly standardized and can be replicated. This makes it possible for other psychologists to replicate the study and hopefully support the findings by obtaining the same results under the same or similar conditions. When this happens, we can say that the research is reliable.
The method can also objective; there Is very little chance for the experimenter's bias and personal opinions to affect the results. The use of single and double-blind methods, as well as the use of technology to assist in measurement, help to guarantee high levels of objectivity.
Experiments yield quantitative data which can be analysed using inferential statistical tests. These tests permit statements to be made about how likely the results are to have occurred by chance.
Case studies: how
When writing about the nature of case study research, there are some key traits of the method that you should be able to discuss.
- Case studies are in-depth studies of an individual or group. They attempt to develop a holistic understanding of an individual's behaviour. This is in contrast to the reductionist approach often taken by biologists.
- Case studies are really a combination of different methods - including physiological measurements, observations, interviews, psychometric testing and brain imaging techniques. This is called method triangulation. In addition, they often use researcher triangulation in which more than one researcher gathers data.
- Case studies are usually longitudinal in nature and thus observe change over time.
- Case studies generate hypotheses to be tested.
- No independent variable is manipulated, so no cause and effect can be determined; however, when several case studies indicate the same trend in behaviour, a cause and effect relationship can be inferred.
Task 3: Thinking about how
Think about Milner's study of HM. This is a classic case study in biological psychology. How does this study demonstrate triangulation of research methods?
1. HM was given psychometric testing - that is, he was given an IQ test. It was found that there was no general intellectual impairment.
2. Interviews were carried out - not just with HM, but with members of this family as well. In addition to method triangulation, this is known as source (or data) triangulation.
3. Observations were carried out. The most well known was learning mirror-drawing. It was observed that he could learn knew behaviours, even if he didn't remember having learned the behaviour.
4. Corkin (1997) carried out an MRI scan on HM and was able to determine the areas of the brain that were damaged.
Case studies: why
Case studies are usually used to help a specific individual or group. In the case of HM, it was about learning how HM's brain worked. In Marmot's study, the goal was to determine how a person's position in a work hierarchy may affect his or her health. Since the situations are rather unique, it is difficult to generalize to a larger population than the one from which the sample was taken.
Case studies are in-depth - giving a lot of data over time. The importance of a case study is that sometimes when research is carried out over time, behaviours may be observed that would not be observed in a short-term study.
Case studies are holistic and often make use of several approaches - also known as theory triangulation. This helps researchers to compensate for the short-comings of a singular method or theoretical framework in trying to understand behave by adopting an integrative approach.