A group of scientists were working on a project to sequence the DNA of an endangered species of gorilla in order to deduce the nucleotide base sequence of a specific gene of interest.
What is the correct process that the scientists may have followed in order to sequence this specific gene from a sample of DNA extracted from a gorilla?
A.
HindIII is added to the sample to cut the DNA on either side of the gene of interest
The double stranded fragments are separated into single strands
Single stranded copies are made using fluorescently labelled nucleotide bases (A, T, C, G)
Capillary electrophoresis is used to separate the DNA by size
B.
A restriction enzyme is added to the sample to cut the DNA on either side of the gene of interest
The double stranded fragments are copied using DNA polymerase
Single stranded copies are made using fluorescently labelled nucleotide bases (A, T, C, G)
Gel electrophoresis is used to separate the DNA by charge
C.
HindIII is added to the sample to cut the DNA on either side of the gene of interest
The double stranded fragments are separated into single strands
Single stranded copies are made using fluorescently labelled nucleotide bases (A, U, C, G)
Capillary electrophoresis is used to separate the DNA by charge
D.
A restriction enzyme is added to the sample to cut the DNA on either side of the gene of interest
Ligase enzyme is used to attach a promotor region to the start of the gene sequence
Single stranded copies are made using fluorescently labelled nucleotide bases (A, U, C, G)
Capillary electrophoresis is used to separate the DNA by size
Cytochrome C is a protein which is common to all organisms and is therefore a useful gene sequence to study when constructing phylogenetic trees to represent evolutionary relationships.
The table shows the number of nucleotide differences in the cytochrome C genes of several different species.
Which of the following shows the correct order of the organisms if arranged from the most closely related to the least closely related compared to humans?
