The pigments in a chloroplast can be separated and identified using a technique called thin layer chromatography. After extracting pigments from a spinach leaf a student carried out thin layer chromatography. Some of their results can be seen below.
Use the equation provided to calculate the Rf values for pigments X and Y.
The effect of different colours of light on the growth of Arabidopsis thaliana (thale cress) seedlings was studied. Three different colours of light were tested and measurements of seedling height, shoot length, and biomass were taken. The results of the study are shown in the table below.
Colour of light
Wavelength of light / nm
Height of seedlings / cm
Shoot length / cm
Total biomass / g
Blue
450
2.3
2.0
2.4
Orange
600
3.5
2.8
2.8
Red
630
7.4
6.1
3.7
State what can be concluded about the effect of different colours of light on the growth of Arabidopsis thaliana from the data shown.
The plant in part b) is an aquatic plant. Before starting the experiment the student conducting the study boiled and then cooled the water that the plant would be placed in.