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Date May 2014 Marks available 2 Reference code 14M.3.sl.TZ2.2
Level SL Paper 3 Time zone TZ2
Command term Calculate and Outline Question number 2 Adapted from N/A

Question

Thin-layer chromatography (TLC) is an example of adsorption chromatography.

A mixture of two organic compounds was separated by TLC using a non-polar solvent.

M14/4/CHEMI/SP3/ENG/TZ2/02.c

(i)     Calculate the Rf values of A and B.

M14/4/CHEMI/SP3/ENG/TZ2/02.c.i

(ii)     Outline why compound B has travelled the greater distance.

Markscheme

(i)     M14/4/CHEMI/SP3/ENG/TZ2/02.c.i/M ;

Award [1] for both correct.

(ii)     B is more soluble in solvent/mobile phase / B is less polar than A / B is less strongly adsorbed onto stationary phase;

Accept B is non-polar.

Do not allow “greater attraction/affinity to solvent” without reference to solubility.

Examiners report

(i)     Very well answered. Many candidates used an inappropriate number of significant figures for the calculated Rf values, but this was not penalized in this instance. 

(ii)     Generally well answered. Some candidates successfully related the solubility of compound B to its polarity.

Syllabus sections

Options » B: Biochemistry » B.2 Proteins and enzymes
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