DNA samples were taken from five individuals and analysed using gel electrophoresis to produce a DNA profile. The DNA profile shows the results for a single gene.
What conclusions can be made from the profile shown in the image?
There are equal frequencies of two alleles
There are 3 different alleles for the gene
There are two alleles and the frequency of one allele is about 75%.
There are three homozygous individuals in the population
In 1989 a company called AquaBounty Technologies genetically engineered (GE) Atlantic salmon to contain a growth hormone-regulating gene which had been extracted from another species of salmon (the Pacific Chinook salmon).
The modification resulted in an increased rate of growth without impacting any other health qualities. Fully grown GE salmon were the same size as the unmodified salmon, however, it took less time for them to reach full size.
Identify which of the following options would not be considered a risk for GE salmon.
There may be changes to the biodiversity of the natural environment
Presence of the new gene may influence the expression of other genes in the genome
There may be a lot of negative publicity surrounding the integration of GE salmon into the human food chain
Some scientists wanted to show the quantity of mRNA that was transcribed from a specific allele of a gene in a sample of cells. The statements explain some of the procedures that the scientists followed to show the presence of the mRNA quantitatively.
Gene probe was added with a coloured dye attached
Reverse transcriptase was added
PCR was carried out to amplify the material
Intensity of colour was studied
Which option gives the correct order for the method followed by the scientists?