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Date May 2011 Marks available 1 Reference code 11M.1.SL.TZ1.19
Level Standard level Paper Paper 1 Time zone Time zone 1
Command term Question number 19 Adapted from N/A

Question

Which enzymes are needed to produce recombinant plasmids to be used in gene transfer?

A. DNA polymerase and DNA ligase
B. DNA polymerase and restriction enzyme (endonuclease)
C. Transcriptase and RNA polymerase
D. Restriction enzyme (endonuclease) and DNA ligase

Markscheme

D

Examiners report

Although this question discriminated well, many candidates chose responses A and B instead of the correct D. It would imply that perhaps many are confusing DNA replication with DNA recombination.

Syllabus sections

Core » Topic 3: Genetics » 3.5 Genetic modification and biotechnology
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