DP Biology Questionbank

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• 16N.3.SL.TZ0.8a: State a suitable fungus for the production of citric acid in the fermenter.
• 16N.3.SL.TZ0.8d: State two uses of the citric acid produced.  
• 16N.3.SL.TZ0.8c: Suggest reasons for the changes in mass of sugar and citric acid after day 6.
• 16N.3.SL.TZ0.8b: Suggest a reason that fermentation is most successful at 30°C.
• 16N.3.HL.TZ0.9b: Outline the effect of mutating the LpxC inhibitor.
• 16N.3.HL.TZ0.9a: Outline the effect of disc 3 on the bacterial lawn.
• 16N.3.HL.TZ0.9c: Predict the results obtained with disc 1 in a Gram-positive bacterial lawn.
• 17M.2.HL.TZ1.1f.ii: Suggest a reason for the greater expression of the gene for the urea transporter after an...
• 17M.3.SL.TZ1.8b: Outline the principles of fermentation by continuous culture.
• 17M.3.SL.TZ1.8a.i: Biogas production in a fermenter requires a substrate. State another requirement for this process.
• 17M.3.SL.TZ1.16a: Calculate the diversity of site C. Working should be shown.
• 17M.3.SL.TZ1.8a.ii: Suggest reasons based on the data in the graph for increases in biogas production at Svensk Biogas.
• 17M.3.HL.TZ2.9c: Distinguish between batch fermentation and continuous fermentation.
• 17M.3.HL.TZ1.8c: Distinguish between the structure of Gram-positive and Gram-negative bacteria. 
• 17M.3.HL.TZ2.9d: Aspergillus niger is used to produce citric acid by continuous fermentation. Glucose is converted...
• 20N.3.SL.TZ0.8c:

  Explain how penicillin can be produced in a fermenter.

• 20N.3.SL.TZ0.8a: Outline a reason for inserting steam into the fermenter before fermentation.
• 20N.3.SL.TZ0.8b: Describe how optimal temperature is maintained inside the fermenter.
• 20N.3.HL.TZ0.10b: Suggest, with a reason, whether this is a batch or a continuous fermentation.
• 20N.3.HL.TZ0.10a.ii:

  Explain the possible causes of these changes in pH.

• 20N.3.HL.TZ0.10c: State one use of the citric acid produced.
• 20N.3.HL.TZ0.10a.i: State in which numbered part of the process you would find the probes to detect changes in pH.
• 17N.3.SL.TZ0.08b:

  Suggest a reason that the fermenter is surrounded by a water jacket.

• 17N.3.SL.TZ0.08c: Identify the waste gas produced.
• 17N.3.SL.TZ0.08a:

  State two conditions in the fermenter that would be monitored by the probes.

   

• 17N.3.SL.TZ0.08d:

  Explain the process of penicillin production in the fermenter.

• 17N.3.HL.TZ0.12b: Temperature is a variable that needs to be continually monitored in deep-tank batch fermentation...
• 17N.3.HL.TZ0.12a:

  Beans contribute to flatulence. Alpha-galactosidase, derived from the fungus Aspergillus niger, is an enzyme that breaks down the fibre usually fermented by bacteria, reducing intestinal gas. Describe how alpha-galactosidase would be produced using A. niger in a continuous fermenter.

• 18N.3.HL.TZ0.10e: Inside Y there are rotating paddles. Outline two reasons for these paddles being needed.
• 18N.3.HL.TZ0.10d: State the main component of biogas.
• 18N.3.HL.TZ0.10b: Deduce, with a reason, whether X is a continuous fermenter or a batch fermenter.
• 18N.3.HL.TZ0.10a: State a group of organisms that will be active in the fermenter labelled X. 
• 18N.3.HL.TZ0.10c: Probes are used to monitor significant variables within the fermenter. List three significant...
• 18M.3.SL.TZ2.8b.iii: Distinguish between batch fermentation and continuous fermentation.
• 18M.3.SL.TZ1.8c: Citric acid can be produced by either batch fermentation or continuous fermentation. Distinguish...
• 18M.3.HL.TZ1.9a.ii: Outline one variable that must be controlled in an industrial fermenter
• 18M.3.SL.TZ2.8a.i: A. aceti is a Gram-negative bacterium. If a Gram staining procedure were carried out on a sample...
• 18M.3.SL.TZ2.8a.ii: A. aceti is a Gram-negative bacterium. If a Gram staining procedure were carried out on a sample...
• 18M.3.SL.TZ1.8a: Identify the nitrogen source that results in the highest yield of citric acid.
• 18M.3.SL.TZ1.8d: Explain the significance of pathway engineering in the industrial use of microorganisms.
• 18M.3.HL.TZ1.9b:

  Explain factors that affect the rate of activity of microorganisms in fermenters.

• 18M.3.HL.TZ1.9a.i: State one fuel that can be produced in this fermenter.
• 18M.3.HL.TZ1.9c: The diagram represents the cell walls of Gram-positive and Gram-negative bacteria. Label the...
• 18M.3.SL.TZ2.8b.ii: Describe one way in which microorganisms in this fermenter could be limited by their own activities.
• 18M.3.SL.TZ1.8b:

  State two uses for industrially produced citric acid.

• 18M.3.SL.TZ2.8b.i: List two abiotic variables that would need to be monitored during this fermentation process.  
• 18M.3.HL.TZ2.10c: Escherichia coli, a Gram-negative bacterium, is a common cause of urinary tract infections. State...
• 19M.3.SL.TZ1.8b: E. coli and P. aeruginosa are both Gram-negative bacteria and S. aureus is Gram-positive. Explain...
• 19M.3.SL.TZ1.8a: Estimate the diameter of the zone of inhibition around the disc containing OPP in the S. aureus...
• 19M.3.SL.TZ1.8c: The three species of bacteria are commonly found on skin. Explain why excessive handwashing with...
• 19M.3.HL.TZ1.9a.iii:

  Suggest a reason for the result with disc X.

• 19M.3.HL.TZ1.9a.i: Estimate the diameter of the zone of inhibition of chloramphenicol.
• 19M.3.HL.TZ1.9b: Explain how it could be determined that B. subtilis is a Gram-positive bacterium.
• 19M.3.HL.TZ1.9a.ii: Distinguish between the action of tetracycline and penicillin on B. subtilis.
• 19M.3.SL.TZ2.8b:

  Using the graph, explain the trend in biogas production over time in the fermenter without pre-treatment.

• 19M.3.SL.TZ2.8a:

  Suggest reasons for the use of a batch culture for the first fermentation and a continuous fermenter for the second fermentation.

• 19M.3.SL.TZ2.8c:

  Suggest one reason for the higher methane content in biogas in pre-treated rice straw waste.

• 19M.3.HL.TZ2.9a.i: State one industrial use of citric acid.
• 19M.3.HL.TZ2.9a.ii:

  State the scientific name (binomial) of the microorganism usually used in this process.

• 19N.3.HL.TZ0.10c: Distinguish between batch and continuous culture fermentation.
• 19N.3.SL.TZ0.8a: Suggest one material that could be loaded into the biogas fermenter from which biogas can be...
• 19N.3.HL.TZ0.10a: Suggest one material that could be loaded into the biogas fermenter from which biogas can be...
• 19N.3.SL.TZ0.8b: Identify the ideal temperature and oxygen conditions inside the fermenter for efficient biogas...
• 19N.3.SL.TZ0.8c: Distinguish between batch and continuous culture fermentation.
• 19N.3.HL.TZ0.10b: Identify the ideal temperature and oxygen conditions inside the fermenter for efficient biogas...
• 19N.3.HL.TZ0.10d: Explain how conditions in the fermenters are maintained to maximize penicillin production.