Increasing carbon dioxide concentration in the atmosphere leads to acidification of the ocean. This in turn reduces the amount of dissolved calcium carbonate. A study was undertaken to investigate the effect of increasing the concentration of atmospheric carbon dioxide on the calcification rate of marine organisms. Calcification is the uptake of calcium into the bodies and shells of marine organisms. The study was undertaken inside Biosphere-2, a large-scale closed mesocosm. The graph shows the results of the data collection.